THE EFFECT OF TEMPERATURE ON THE ACTIVITY OF BACILLUS SPECIES IN BIOREMEDIATION OF PALM OIL EFFLUENT
Abstract
This study investigated the effect of temperature on the bioremediation of palm oil mill effluent contaminated soil by Bacillus species. The physicochemical parameters of the palm oil mill effluents were temperature (3 3.5°C), Total Dissolved Solids (465.5 mg/I), Total Soluble Solids (310 mg/I), Dissolved Oxygen (0.162 mg/l), Biological Oxygen Demand (0.13 mg/i), Chemical Oxygen Demand (123.5mg/l). The Bacillus species was isolated from pam oil mill effluent contaminated soil samples in Tsoaka Village, Bida, Niger State, Nigeria. The 3rnl culture of the isolate was inoculated into medium comprising 2g of yeast extract, 2g of glucose, 2.8g of lactose broth in 50 mi of different concentrations of the effluent (0%, 5%, 10%, 30%, 50%, 70%, 90% and 100 %) and incubated for 30 days at 37°C. The effects of temperature (30°C, 35°C, 40°C, 45°C) on the remediation potential of the isolates were determined. The rate of bioremediation was determined using spectrophotometry at 600 nm. The optimum bioremediation temperature was 35°C (60.7%) after 30 days. The results obtained in this study revealed that 35°C was optimum for remediation by Bacillus species of palm oil mill effluent contaminated soil.
Keywords
Bacillus species, Palm Oil Effluents, Biodecolourisation, Spectrophotometry