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DETERMINATION OF XYLANASE ACTIVITY USING STREPTOMYCES, ISOLATED FROM KALGO FADAMA LAND SOIL, KEBBI STATE, NIGERIA

Abstract

Xylanase is the most important enzymes that is currently being used in a number of industries. Xylanases hydrolyze the reprecipitated xylan on the fibers of paper pulp. The permeability of fibers increases with xylanase treatment, which allows easier removal of lignin from the fibres. The aim of this research is to determine the activity of xylanase enzyme using soil Streptomyces collected from Dangwarema Fadama area of kalgo local government, Kebbi State, Nigeria. The soil sample was pre-treated to eliminate the commonly found microbes using physic-chemical methods. After inoculation, Streptomyces showing distinct morphological characteristics were selected from the isolation plate and further sub-cultured to obtain the pure form of the isolate, and then inoculated into production media for the production of the enzyme. The best Corbin sources and incubation period were determined. During the process of isolation, identification of the colonies with different morphology such as large white colonies with fluffy spores, small white sporulation powdery, colonies producing light brown pigment were determined. The xylanase was produced by isolated bacteria, and then optimization of time and carbon source were determined by measuring the enzyme activity. For time optimization, the highest activity of (0.512umol) was recorded at 32hrs followed by 28hrs (0.155umol), and then 24hrs (0.142umol) which is the lowest, and according to this research glucose was the best carbon source compared to other carbon sources. It can be concluded that the isolated bacteria (Streptomyces) from Kalgo Fadama land soil is capable of producing microbial xylanase enzyme. And according to this research, the best carbon source and best incubation period of xylanase enzyme were glucose and 32hrs respectively.

Keywords

Xylanase, Enzyme, Production, Activity

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